tRNA–guanine transglycosylase from E. coli: a ping-pong kinetic mechanism is consistent with nucleophilic catalysis
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چکیده
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Pyruvate carboxylase from chicken liver. Steady state kinetic studies indicate a "two-site" ping-pong mechanism.
On the basis of initial velocity and product inhibition studies a nonclassical Ping Pong Bi Bi Uni Uni mechanism has been proposed for pyruvate carboxylase from chicken liver. The nonclassical feature of this mechanism is the proposal that each active site on the enzyme is composed of two separate and functionally distinct catalytic sites, i.e., a separate catalytic site exists for the reactant...
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An efficient enzyme kinetics assay using electrospray ionization mass spectrometry (ESI-MS) was initially applied to the catalytic mechanism investigation of a carbohydrate sulfotransferase, NodST. Herein, the recombinant NodST was overexpressed with a His(6)-tag and purified via Ni-NTA metal-affinity chromatography. In this bisubstrate enzymatic system, an internal standard similar in structur...
متن کاملproceed via the Ping-Pong mechanism
Acyl-transfer catalysed by y-glutamyltranspeptidase from bovine kidney was studied using y-Land y-D-Glu-p-nitroanilide as the donor and GlyGly as the acceptor. The transfer of the y-Glu group to GlyGly was shown to be accompanied by transfer of the y-Glu group to water (hydrolysis). The results were compared with acyl-transfer catalysed by the representative serine protease, a-chymotrypsin. The...
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An efficient enzyme kinetics assay using electrospray ionization mass spectrometry (ESI-MS) was initially applied to the catalytic mechanism investigation of a carbohydrate sulfotransferase, NodST. Herein, the recombinant NodST was overexpressed with a His6-tag and purified via Ni-NTA metal-affinity chromatography. In this bisubstrate enzymatic system, an internal standard similar in structure ...
متن کاملA ping-pong ball in left atrium.
Accepted 18 December 2017 DesCripTion Rheumatic heart disease (RHD) is much prevalent in low/middle-income country like India with the prevalence ranging from 0.2 to 1.1/1000 for RHD and from 0.0007 to 0.2/1000 for rheumatic fever. Factors that precipitate the formation of clot are atrial fibrillation (AF) rhythm, left atrial (LA) size, duration of symptoms, advanced age and severity of mitral ...
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ژورنال
عنوان ژورنال: Bioorganic Chemistry
سال: 2003
ISSN: 0045-2068
DOI: 10.1016/s0045-2068(03)00069-5